Properties:
RelatedCategories | Analytical/Chromatography,HPLC,HPLCColumns,byBrandforSmallMolecules,apHeraC4Reversed-phaseColumns,apHeraPolymericHPLCcolumns,highpHapplications |
Description:
Generaldescription
apHera™reversedcolumnsweredevolopedspecificallytoprovidethesuperioradvantagesofbothsilicaandpolystyrenecolumns,withoutthedisadvantagesofeither.Thiswasaccomplishedusingavinylalcoholcopolymerbasethatkeepsthesurfacewettedevenwithhighcarbonloads.Theporousstructurehasanaverageporediameterlargeenoughtoproduceidealresultsforsmallanalytes,peptidesandsmallproteins.ThesecolumnequalsilicabasedcolumnsinseparationefficiencywithorganicsolventsbutprovideefficiencywithbufferedalkalinesolutionsnotpossIBLeonsilica.Oneofthemostsignificantfeaturesinlogicialelutionorderofalkylatedbaseswhereretentionincreasesproportionatelywithincreasingchainlength.
FeaturesofapHera™ReversedPhaseColumns
• Stablevinylcopolymerbase
• StablepHrange2-12
• 300angstromporesize
• Stableinallorganicsolvents
• Expandedapplications:peptides,proteins
• Efficientseparationofanybasicsubstance
• AmenABIlitytowashingandalkalinesolutions
apHera™reversedcolumnsweredevolopedspecificallytoprovidethesuperioradvantagesofbothsilicaandpolystyrenecolumns,withoutthedisadvantagesofeither.Thiswasaccomplishedusingavinylalcoholcopolymerbasethatkeepsthesurfacewettedevenwithhighcarbonloads.Theporousstructurehasanaverageporediameterlargeenoughtoproduceidealresultsforsmallanalytes,peptidesandsmallproteins.Thesecolumnsequalsilicabasedcolumnsinseparationefficiencywithorganicsolventsbutprovideefficiencywithbufferedalkalinesolutionsnotpossibleonsilica.Oneofthemostsignificantfeaturesislogicialelutionorderofalkylatedbaseswhereretentionincreasesproportionatelywithincreasingchainlength.
FeaturesofapHera™ReversedPhaseColumns
• Stablevinylcopolymerbase
• StablepHrange2-12
• 300angstromporesize
• Stableinallorganicsolvents
• Expandedapplications:peptides,proteins
• Efficientseparationofanybasicsubstance
• Amenabilitytowashingandalkalinesolutions
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LegalInformation
apHeraisatrademarkofSigma-AldrichCo.LLC