Synonym:
Mucopeptide N-acetylmuramoylhydrolase,
Muramidase
Properties:
Related Categories |
3.2.x.x Glycosidases,
3.x.x.x Hydrolases,
Amino Acid, Peptide & Protein Standards,
Analytical Standards,
Analytical/Chromatography,
|
grade
|
analytical standard
|
product line
|
VETRANAL™
|
form
|
neat
|
shelf life
|
limited shelf life, expiry date on the label
|
mol wt
|
single-chain mol wt 14.3 kDa
|
|
Mr ~14000
|
application(s)
|
HPLC: suitable
|
|
gas chromatography (GC): suitable
|
format
|
neat
|
storage temp.
|
2-8°C
|
Description:
Application
Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Biochem/physiol Actions
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Legal Information
VETRANAL is a trademark of Sigma-Aldrich Co. LLC
Safety:
Personal Protective Equipment
Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RIDADR
NONH for all modes of transport