Description
ThermoScientificPageRulerPrestainedProteinLadderisamixtureoften(10)blue-,orange-andgreen-stainedproteins(10to180kDa)foruseassizestandardsinproteinelectrophoresis(SDS-PAGE)andWesternblotting.
FeaturesofPageRulerPrestainedProteinLadder:
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Sizerange—10proteinsspanning10to180kDa
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Ready-to-use—suppliedinalo
ADIngbufferfordirectloadingongels;noneedtoboil
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Sharpbands—color-codedbandsofsimilarintensityforeasyvisualization
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Qualitytested—eachlotevaluatedbySDS-PAGEandWesternblotting
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Tworeferencebands—orangeat70kDaandgreenat10kDa
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Membrane-compatIBLe—coloredbandstransfertomembranesforWesternblotting
ThisprestainedproteinMW
MarkerisdesignedformonitoringtheprogressofSDS-polyacrylamidegelelectrophoresis,forassessingtransferefficiencyontoPVDF,nylonandnitrocellulosemembranes,andforestimatingtheapproximatesizeofseparatedproteinsthathavebeenmadevisiblewithgelstainsorWesternblotdetectionreagents.Theladdercontainsoneorangereferencebandat70kDaandonegreenbandat10kDa.
Includes:•Dye-stainedproteinsin62.5mMTris-H3PO4(pH7.5at25°C),1mMEDTA,2%SDS,10mMDTT,1mMNaN3and33%glycerol.
Applications:•MonitoringproteinmigrationduringSDS-polyacrylamidegelelectrophoresis
•MonitoringproteintransferontomembranesafterWesternblotting
•SizingofproteinsonSDS-polyacrylamidegelsandWesternblots
ForResearchUseOnly.Notforuseindiagnosticprocedures.