This stain is useful in demonstrating atrophy of elastic tissue in cases of emphysema, thinning and loss of elastic fibers in arteriosclerosis and other vascular diseases.
500ml each of the following:
- Solution A - 5% Alcoholic Hematoxylin
- Solution B - 10% Aqueous Ferric Chloride
- Solution C - Iodine Solution
- Solution D - 5% Sodium Thiosulphate
- Solution E - Counterstain I
- Solution F - Counterstain II