This is a combined method utilizing the properties of both PAS and Alcian blue pH 2.5 methods to demonstrate the full complement of tissue proteoglycans. Alcian Blue pH 2.5 stains the acid mucins blue while PAS stains the neutral mucins pink to red.
Stains up to 200 slides
Kit Contains: 500 ml each:
Alcian Blue (1% in 3% Acetic Acid)
Schiff"s Reagent
0.5% Periodic Acid
Harris Hematoxylin
Scott"s Bluing
Results:
Acid mucins- blue
Neutral mucins and other PAS positive tissue elements- pink to red