HOPE® Formalin-free fixation technique for molecular pathology and research.
Tissue conservation using the HOPE® technique provides paraffin embedded tissue blocks with native structure of the macromolecules (protein, DNA, RNA) in the tissue. (Hepes-Glutamic acid buffer mediated Organic solvent Protection Effect)
During HOPE® fixation, tissue samples are sequentially incubated in HOPE® I and HOPE® II solutions, followed by a dehydration with acetone and subsequent paraffin embedding. No alcohols or aldehydes (formalin, glutaraldehyde) are used in this tissue processing method. As a consequence, there is no cross linking of macro molecular structures during the process. This opens up new opportunities to characterize tissues out of paraffin blocks.
Many "cryo-type" antibodies are compatible with HOPE® fixed tissue sections, and many antibodies additionally work without any heat pretreatment or proteolytic digestion steps often necessary for formalin fixed tissue sections.
When keeping the tissue specimen in the fixation solution there is no "over-fixation" with HOPE®, as is a known occurance with formalin. This results in a more standardized fixation process.
RNA, DNA and proteins are conserved in high quality, thus making subsequent applications out of the same tissue block possible such as PCR, RT-PCR, northern-blot, immunohistochemistry, western-blot and many others.
Kit Contains: 10 transportation vials filled with 5 ml HOPE® I with protection vials, 1 x 1 ml HOPE® II and 200 grams low melt point paraffin.
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