Gomori’s One Step Trichrome refers to the multiple stain reaction of this reagent only. However, adequate staining of all cell components requires several other solutions to obtain balance in the tissue. The mordant with Bouin’s Fixative is used to drop the pH and for protein interaction in the section. While this mechanism is not clear the stain works best with this step included.
Kit Components:
Bouin’s Fixative(250 ml)
Weigert’s Iron Hematoxylin Solution A & B(250 ml/ each)