當(dāng)前位置 ： Millipore >>> Millipore/17-486 | phospho-IκB (Ser32) STAR ELISA Kit/17-486/96 assays
Millipore/17-486 | phospho-IκB (Ser32) STAR ELISA Kit/17-486/96 assays
  • Millipore/17-486 | phospho-IκB (Ser32) STAR ELISA Kit/17-486/96 assays

Millipore/17-486 | phospho-IκB (Ser32) STAR ELISA Kit/17-486/96 assays

價(jià)格: ￥6900.00 市場(chǎng)價(jià): 11500.00
貨號(hào): 17-486
品牌: Millipore
規(guī)格
數(shù)量
庫(kù)存(0)
特別 提示
代購(gòu)產(chǎn)品：無(wú)質(zhì)量問題不接受退換貨，下單前請(qǐng)仔細(xì)核對(duì)信息。
下單后請(qǐng)及時(shí)聯(lián)系客服核對(duì)商品價(jià)格，訂單生效后再付款。
資深產(chǎn)品顧問
咨詢顧問

全國(guó)免費(fèi)服務(wù)熱線

4000-520-616

  • 自營(yíng)商城 一站式服務(wù)
  • 廠家直采 剔除溢價(jià)
  • 品質(zhì)甄選 正品保證
  • 嚴(yán)控流程 只做188精品
  • 極速物流 如約送貨
  • 詳情
  • 使用說明
  • 常見問題
    • Description
      CatalogueNumber17-486
      BrandFamilyUpstate
      TradeName
      • STAR
      • Upstate
      Descriptionphospho-IκB(Ser32)STARELISAKit
      BackgroundInformationI.TESTPRINCIPLE:
      ThecolorimetricSTAR(SignalTransductionAssayReaction)ELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtodetectspecificlevelsofsignalingtargetsinwholecellextracts.TheIκBplateiscoatedwithaspecificmousemonoclonalIκBcaptureantibodyonthemicrowellsofthe96-wellclearplate.SamplelysateorthestandardincludedinthekitareincubatedinthemicrowellsallowingIκBantigentobecapturedintheplatewells.Theplateisthenwashedtoremoveanyun-boundnonspecificmaterial.Aspecificrabbitanti-phospho-IκBantibodyisaddedtodetectthecapturedIκBontheplatewellthatisphosphorylatedonSer32.TheunbounddetectionantibodyiswashedawayfollowedbyincubationwithanHRP-conjugatedanti-rabbitantibody.Thisallowsforasensitiveenzymaticdetectionofthesample.AftertheadditionofTMBsubstrateandstopsolutiontheabsorbanceismeasuredat450nmusingaplatereader.
      Theentireassaytakeslessthan5hourstocompletewithminimalhands-ontime.Manyofthereagentsaresuppliedinready-touseformulationsforeaseofuse.ThekitalsoincludesastandardthatisrunasbothapositivecontrolandtogenerateastandardcurveforIκBmeasurement.

      II.BACKGROUND:
      NFκBsignalingisacriticalregulatornotonlyofimmunefunction,butalsoofproliferationversusapoptosisinresponsetovariousstimuli.Inamajorityofunstimulatedcells,theNFκBtranscriptionfactorsexistintheirinactiveformandareretainedinthecytoplasmbytheboundinhibitoryIκBproteinsthatpreventitfromenteringthenucleus,thuskeepingNFκBinaninactivestate.Uponstimulationbymultipleinducersincludingvirusesorcytokines,suchasTNFα,IL-1,orPMA,IκBαisrapidlyphosphorylatedbyIKK(IκBKinase),whichphosphorylatesIκBonserines32and36,allowingrecognitionbytheubiquitinligasemachinery,leADIngtoitspolyubiquinationanddegradation,resultinginthereleaseoftheNFκBcomplex.OnceIκBisdegraded,NFκBisabletoinitiatetranscription.TheNFκBcomplexthentranslocatestothenucleuswhereitactivatesgenetranscription.NFκBinducesthetranscriptionofitsowninhibitor,IκBα,causinganautoregulatorymechanismofNFκBactivityandgeneratingtheinactiveformofNFκB.ThenewlyformednuclearNFκB-IκBαcomplexesarethenexportedouttothecytoplasm,therebyreestablishingthecytoplasmicpoolofinactiveNFκBcomplexesprimedforanotherroundofactivationtotakeplace.ThewidevarietyofgenesregulatedbyNFκBincludesthoseencodingcytokines,chemokines,adhesionmolecules,acutephaseproteins,andinducIBLeeffectorenzymes.MutantIκBinwhichserines32and36arechangedtoalanines,isnotphosphorylated,andthereforenotdegraded.CellsexpressingthisproteinarenotabletoactivateNFκB,providingausefultooltostudytheroleofNFκBinvariouspathwaysandprocesses.
      MaterialsRequiredbutNotDelivered1.Multi-channelorrepeatingPipettes
      2.Plateshaker(optional)
      3.Pipettors&tipscapableofaccuratelymeasuring1-1000μL
      4.GraduatedSEROlogicalpipettes
      5.96-wellmicrotiterPlateReaderwith450nmfilter
      6.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata
      7.Microfugetubesforstandardandsampledilutions
      8.Mechanicalvortex
      9.1litercontainer
      10.Distilledordeionizedwater
      ProductInformation
      Components
      • 1.CapturePlatepre-coatedwithanti-IκBantibody:(PartNo.17-486A)Onepre-coated96-stripwellimmunoplatesealedinafoilpouch.
      • 2.Anti-phospho-IκB(ser32)detectionantibody:(PartNo.17-486B)Onebottle(6mL)ofanti-phospho-IκB(Ser32)detectionantibodycontainingsodiumazide,readytouse.
      • 3.ELISADiluent:(PartNo.17-486C)Onebottle(25mL)ofELISADiluentcontainingsodiumazide,readytouse.
      • 4.25XELISAWashBuffer:(PartNo.17-486D)Onebottle(50mL)of25XELISAWashBuffer.
      • 5.Anti-RabbitIgGHRPconjugate:(PartNo.17-486E)Onevial(125μL)of100Xanti-rabbitHRPconjugate.
      • 6.HRPDiluent:(PartNo.17-486F)Onebottle(25mL)ofHRPDiluent.
      • 7.TMBSolution:(PartNo.17-486G)Onebottle(25mL)ofstABIlizedtetramethylbenzidine(TMB),readytouse.
      • 8.StopSolution:(PartNo.17-486H)Onebottle(25mL)ofstopsolution,readytouse.
      • 9.PhosphorylatedIκB(Ser32)Standard:(PartNo.17-486I)TwovialsofphosphorylatedIκBstandard(Ser32),lyophilized.
      • 10.PlateCovers:Twoplatecovers.
      DetectionmethodColorimetric
      StorageandShippingInformation
      StorageConditions1yearat4°Cfromdateofshipment
      Applications
      ApplicationThisphospho-IκB(Ser32)STARELISAKitisusedtomeasure&quantifyIκBlevels.
      KeyApplications
      • ELISA
      ApplicationNotes?Allkitreagentsshouldbebroughttoroomtemperature(20°Cto25°C)priortouse.
      ?Donotusereagentsbeyondtheexpirationdateofthekit.
      ?Donotmixorinterchangereagentfromvariouskitlots.
      ?ManualPlateWashing:Vigorouswashingandcompleteremovalofallliquidbyaspirationattheendofeachwashingstepisveryimportanttoobtainlowbackgroundvalues.Gentleagitationduringthewashstepsora2-3minutesoakmayreducebackgroundvalues.
      ?Thedesiccantenclosedinthe96-wellcaptureplatepouchwillkeeptheplatestablewhenstoredat2°to8°Cshouldthepouchlooseitssealduringshipping.
      BIOLOGicalInformation
      SpeciesReactivity
      • Human
      AnalytesAvailable
      • IκB
      EntrezGeneNumber
      EntrezGeneSummaryNFKB1(MIM164011)orNFKB2(MIM164012)isboundtoREL(MIM164910),RELA(MIM164014),orRELB(MIM604758)toformtheNFKBcomplex.TheNFKBcomplexisinhibitedbyI-kappa-Bproteins(NFKBIAorNFKBIB,MIM604495),whichinactivateNF-kappa-Bbytrappingitinthecytoplasm.PhosphorylationofserineresiduesontheI-kappa-Bproteinsbykinases(IKBKA,MIM600664,orIKBKB,MIM603258)marksthemfordestructionviatheubiquitinationpathway,therebyallowingactivationoftheNF-kappa-Bcomplex.ActivatedNFKBcomplextranslocatesintothenucleusandbindsDNAatkappa-B-bindingmotifssuchas5-primeGGGRNNYYCC3-primeor5-primeHGGARNYYCC3-prime(whereHisA,C,orT;RisanAorGpurine;andYisaCorTpyrimidine).[suppliedbyOMIM]
      GeneSymbol
      • NFKBIA
      • IKB-alpha
      • IKBA
      • I-kappa-B-alpha
      • MAD-3
      • MAD3
      • IkappaBalpha
      • NFKBI
      Modifications
      • Phosphorylation
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P25963#InhibitsNF-kappa-Bbycomplexingwithandtrappingitinthecytoplasm.MaybeinvolvedinregulationoftranscriptionalresponsestoNF-kappa-B,includingcelladhesion,immuneandproinflammatoryresponses,apoptosis,differentiationandgrowth.Controlledbysequentialserine-phosphorylation,ubiquitinationanddegradation.Tyrosine-phosphorylationcouldonlyleadtodissociationfromNF-kappa-B.
      SIZE:317aminoacids;35609Da
      SUBUNIT:Interactswithp65(RELA).InteractswithNKIRAS1andNKIRAS2.InteractswithHBVproteinX.
      SUBCELLULARLOCATION:Cytoplasm.
      PTM:Phosphorylated;disablesinhibitionofNF-kappa-BDNA-bindingactivity.&Ubiquitinated;subsequenttostimulus-dependentphosphorylationonserines,polyubiquitinationtargetstheproteinforrapiddegradationviatheubiquitinsystem.
      DISEASE:SwissProt:P25963#DefectsinNFKBIAareacauseofautosomaldominantanhidroticectodermaldysplasiawithimmunodeficiency(AD-EDA-ID)[MIM:164008].Ectodermaldysplasias(EDs)constituteaheterogeneousgroupofdevelopmentaldisordersaffectingtissuesofectodermalorigin.EDsarecharacterizedbyabnormaldevelopmentoftwoormoreectodermalstructuressuchashair,teeth,nailsandsweatglands,withorwithoutanyadditionalclinicalsign.Eachcombinationofclinicalfeaturesrepresentsadifferenttypeofectodermaldysplasia.
      SIMILARITY:SwissProt:P25963##BelongstotheNF-kappa-Binhibitorfamily.&Contains5ANKrepeats.
      PhysicochemicalInformation
      Sensitivity
      • Sensitivity:1Unit/mL.
        RangeofDetection:1.6to100Units/mL
      Dimensions
      MaterialsInformation
      MaterialsInformation
    售后保障
    螞蟻淘生物188，秉承螞蟻淘一貫的嚴(yán)謹(jǐn)態(tài)度，由螞蟻淘公司專業(yè)人員負(fù)責(zé)品控、采購(gòu)、物流、銷售、售后，保障正品優(yōu)質(zhì)。以“快速好省，為科研提供好產(chǎn)品、好價(jià)格”為理念，直接鏈接原廠家，從全國(guó)各地原制造商嚴(yán)格挑選188款科研精品，剔除品牌溢價(jià)，188生物新電商，把好的產(chǎn)品帶給科研！? 力求給你最優(yōu)質(zhì)的商品。
  • Q：生物188產(chǎn)品正品保障嗎？
    A：生物188質(zhì)量把控人員具有十年的從業(yè)經(jīng)驗(yàn)，在業(yè)界享有良好的口碑；自營(yíng)商城，直接從廠家采購(gòu)， 自己的團(tuán)隊(duì)負(fù)責(zé)國(guó)際物流和清關(guān)，中間沒有第三方，所有流程嚴(yán)格把控，100%保證正品，假一罰十。

    Q：下單后可以修改訂單嗎？
    A：下單后的商品付款之前可以修改；訂單付款成功，需要聯(lián)系我們客服進(jìn)行修改；客服電話：4000-520-616

    Q：可以開發(fā)票嗎？
    A：本網(wǎng)站所售商品都是正規(guī)清關(guān)，均開具16%正規(guī)發(fā)票，發(fā)票金額含配送費(fèi)金額，另有說明的除外。

    Q：商品幾天可以發(fā)貨？
    A：生物188商品，全部現(xiàn)貨銷售，付款后即可發(fā)貨,一般一周內(nèi)送達(dá)！

    Q：如何聯(lián)系商家？
    A：有任何問題夠可以電話咨詢我們，全國(guó)24小時(shí)免費(fèi)服務(wù)熱線：4000-520-616 或聯(lián)系我們的在線客服QQ：1570468124

    Q：收到的商品少了/發(fā)錯(cuò)了怎么辦？
    A：同個(gè)訂單購(gòu)買多個(gè)商品可能會(huì)分為一個(gè)以上包裹發(fā)出，可能不會(huì)同時(shí)送達(dá)，建議查看訂單詳情是否是 部分發(fā)貨狀態(tài)；如未收到，可聯(lián)系在線客服或者致電4000-520-616。

    Q：退換貨/維修需要多長(zhǎng)時(shí)間？
    A：一般情況下，退貨處理周期為客戶收到產(chǎn)品一個(gè)月內(nèi)（以快遞公司顯示簽收時(shí)間為準(zhǔn)），包裝規(guī)格、 數(shù)量、品種不符，外觀毀損、短缺或缺陷，請(qǐng)?jiān)谑盏截?4小時(shí)內(nèi)申請(qǐng)退換貨；特殊商品以合同條款為準(zhǔn)。

何為188

極簡(jiǎn)而嚴(yán)謹(jǐn)，我們僅銷售188款生物醫(yī)學(xué)科研用品，款款都是爆款；因?yàn)樯偎跃劢?，聚焦甄選每一款產(chǎn)品，聚焦服務(wù)每一位客戶!

關(guān)注我們 :

點(diǎn)擊QQ聯(lián)系我們
生物188微信

關(guān)注188微信公眾號(hào)

獲取最新優(yōu)惠活動(dòng)通知

  • 品質(zhì)甄選，正品保證

  • 自營(yíng)電商，廠家直采

  • 極簡(jiǎn)主義，188精品