當前位置 ： Millipore >>> Millipore/17-10054 | ChIPAb+ Histone H2B - ChIP Validated Antibody and Primer Set/17-10054/25 assays25 assays per set. R
Millipore/17-10054 | ChIPAb+ Histone H2B - ChIP Validated Antibody and Primer Set/17-10054/25 assays25 assays per set. R
  • Millipore/17-10054 | ChIPAb+ Histone H2B - ChIP Validated Antibody and Primer Set/17-10054/25 assays25 assays per set. R

Millipore/17-10054 | ChIPAb+ Histone H2B - ChIP Validated Antibody and Primer Set/17-10054/25 assays25 assays per set. R

價格: 試用 市場價: 0.00
貨號: 17-10054
品牌: Millipore
規(guī)格
數量
庫存(0)
特別 提示
代購產品：無質量問題不接受退換貨，下單前請仔細核對信息。
下單后請及時聯(lián)系客服核對商品價格，訂單生效后再付款。
資深產品顧問
咨詢顧問

全國免費服務熱線

4000-520-616

  • 自營商城 一站式服務
  • 廠家直采 剔除溢價
  • 品質甄選 正品保證
  • 嚴控流程 只做188精品
  • 極速物流 如約送貨
  • 詳情
  • 使用說明
  • 常見問題
    • Description
      CatalogueNumber17-10054
      BrandFamilyUpstate
      TradeName
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+HistoneH2B-ChIPValidatedAntibodyandPrimerSet
      OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
      TheChIPAb+HistoneH2BsetincludestheHistoneH2Bantibody,anegativecontrolrabbitIgG,andqPCRprimerswhichamplifya110bpregionofhumanβ-globinpromoter.TheHistoneH2Bandnegativecontrolsaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofHistoneH2B-associatedchromatin.
      AlternateNames
      • H2B
      • HistoneH2B
      • H2Bhistonefamily,memberQ
      • histone2,H2be
      • histonecluster2,H2be
      BackgroundInformationHistoneH2Bisoneofthe5mainhistoneproteinsinvolvedinthestructureofchromatinineukaryoticcells.FeaturingamainglobulardomainandalongNterminaltailH2Aisinvolvedwiththestructureofthenucleosomesofthe"beadsonastring"structure.
      ProductInformation
      FormatPurified
      Control
      • IncludesnegativecontrolnormalrabbitIgGantibodyandprimersspecificforhumanβ-globin.
      PresentationAnti-HistoneH2B(rabbitpolyclonal).Onevialcontaining25μLofproteinApurifiedrabbitpolyclonalinbuffercontaining0.02Mphosphatebuffer,pH7.6,0.25MNaCl,and0.1%sodiumazide,and30%glycerol.Storeat-20°C.

      NormalRabbitIgG.Onevialcontaining125μgofpurifiedrabbitIgGin125μLstoragebuffer.Storeat-20°C.

      ChIPPrimers,β-globin.Onevialcontaining75μLof5μMofeachprimerspecificforthehumanβ-globinpromoter.Storeat-20°C.
      FOR:AGGACAGGTACGGCTGTCATC
      REV:TTTATGCCCAGCCCTGGCTC
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIllityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
      Applications
      ApplicationTheChIPAb+HistoneH2BsetincludestheHistoneH2Bantibody,anegativecontrolrabbitIgG&qPCRprimerswhichamplifya110bpregionofhumanβ-globinpromoter.
      KeyApplications
      • WesternBlotting
      • ChromatinImmunoprecipitation(ChIP)
      ApplicationNotesChromatinImmunoprecipitation:
      SonicatedchromatinpreparedfromHeLacells(1X106cellequivalentsperIP)weresubjectedtochromatinimmunoprecipitationusingeither1μLofNormalRabbitIgGor1μLofAnti-HistoneH2BandtheMagnaChIP?AKit(Cat.#17-610).
      SuccessfulimmunoprecipitationofHistoneH2BassociatedDNAfragmentswasverifiedbyqPCRusingChIPPrimers,humanβ-globinforapositive,andhumanGAPDHprimersasanegativeassay(Pleaseseefigures).
      DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated.
      PleaserefertotheEZ-MagnaChIP?A(Cat.#17-408)orEZ-ChIP?(Cat.#17-371)protocolforexperimentaldetails.

      WesternBlotAnalysis:
      Representativelotdata.
      RecombinantXenopusHistoneH2B,chickencorehistonesandacidextractfromeithersodiumbutyrateorcolcemidtreatedHeLacellswereresolvedbyelectrophoresis,transferredtonitrocelluloseandprobedwithanti-HistoneH2B(1:5,000dilution).
      Proteinswerevisualizedusingagoatanti-rabbitsecondaryantibodyconjugatedtoHRPandachemiluminescencedetectionsystem(Pleaseseefigures).
      BiologicalInformation
      ImmunogenKLH-conjugated,syntheticpeptidecorrespondingtoaminoacids118-126(CG-AVTKYTSSK)ofhumanHistoneH2B,withanN-terminalCGaddedforconjugationpurposes.
      HostRabbit
      SpecificityRecognizesandisspecificforHistoneH2B,MW~15kDa.Anadditionalbandat~23kDaisdetectedinsomesamples,whichlikelycorrespondstoubiquityl-HistoneH2B.
      SpeciesReactivity
      • Chicken
      • Xenopus
      • Human
      • Yeast(S.cerevisiae)
      SpeciesReactivityNoteHuman,chicken,yeastandXenopus.Othersnottested.Broadspeciescross-reactivityexpectedbasedonsequencehomology.
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneisintronlessandencodesamemberofthehistoneH2Bfamily.Transcriptsfromthisgenecontainapalindromicterminationelement.
      GeneSymbol
      • GL105
      • H2B
      • H2B-GL105
      • H2B.1
      • H2B.q
      • H2B/q
      • H2BFQ
      • MGC119802
      • MGC119804
      • MGC129733
      • MGC129734
      • OTTHUMP00000013920
      PurificationMethodProteinAPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q8N257#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.
      SIZE:126aminoacids;13908Da
      SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.
      SUBCELLULARLOCATION:Nucleus.
      PTM:MonoubiquitinationofLys-121bytheRNF20/40complexgivesaspecifictagforepigenetictranscriptionalactivationandisalsoprerequisiteforhistoneH3"Lys-4"and"Lys-79"methylation.ItalsofunctionscooperativelywiththeFACTdimertostimulateelongationbyRNApolymeraseII.&PhosphorylatedonSer-15bySTK4/MST1duringapoptosis;whichfacilitatesapoptoticchromatincondensation.AlsophosphorylatedonSer-15inresponsetoDNAdoublestrandbreaks(DSBs),andincorrelationwithsomatichypermutationandimmunoglobulinclass-switchrecombination.
      SIMILARITY:SwissProt:Q8N257##BelongstothehistoneH2Bfamily.
      MolecularWeight~15kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
    售后保障
    螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態(tài)度，由螞蟻淘公司專業(yè)人員負責品控、采購、物流、銷售、售后，保障正品優(yōu)質。以“快速好省，為科研提供好產品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產品帶給科研！? 力求給你最優(yōu)質的商品。
  • Q：生物188產品正品保障嗎？
    A：生物188質量把控人員具有十年的從業(yè)經驗，在業(yè)界享有良好的口碑；自營商城，直接從廠家采購， 自己的團隊負責國際物流和清關，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

    Q：下單后可以修改訂單嗎？
    A：下單后的商品付款之前可以修改；訂單付款成功，需要聯(lián)系我們客服進行修改；客服電話：4000-520-616

    Q：可以開發(fā)票嗎？
    A：本網站所售商品都是正規(guī)清關，均開具16%正規(guī)發(fā)票，發(fā)票金額含配送費金額，另有說明的除外。

    Q：商品幾天可以發(fā)貨？
    A：生物188商品，全部現(xiàn)貨銷售，付款后即可發(fā)貨,一般一周內送達！

    Q：如何聯(lián)系商家？
    A：有任何問題夠可以電話咨詢我們，全國24小時免費服務熱線：4000-520-616 或聯(lián)系我們的在線客服QQ：1570468124

    Q：收到的商品少了/發(fā)錯了怎么辦？
    A：同個訂單購買多個商品可能會分為一個以上包裹發(fā)出，可能不會同時送達，建議查看訂單詳情是否是 部分發(fā)貨狀態(tài)；如未收到，可聯(lián)系在線客服或者致電4000-520-616。

    Q：退換貨/維修需要多長時間？
    A：一般情況下，退貨處理周期為客戶收到產品一個月內（以快遞公司顯示簽收時間為準），包裝規(guī)格、 數量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內申請退換貨；特殊商品以合同條款為準。

何為188

極簡而嚴謹，我們僅銷售188款生物醫(yī)學科研用品，款款都是爆款；因為少所以聚焦，聚焦甄選每一款產品，聚焦服務每一位客戶!

關注我們 :

點擊QQ聯(lián)系我們
生物188微信

關注188微信公眾號

獲取最新優(yōu)惠活動通知

  • 品質甄選，正品保證

  • 自營電商，廠家直采

  • 極簡主義，188精品