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Pierce/Dynabeads M-280 Tosylactivated/2 mL/14203
  • Pierce/Dynabeads M-280 Tosylactivated/2 mL/14203

Pierce/Dynabeads M-280 Tosylactivated/2 mL/14203

價格: ¥4272.00 市場價: 7120.00

貨號: 14203
品牌: Pierce
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    • Description

      Whenyouwishtocovalentlycoupleantibodies,peptides,intactproteinsandfunctionalenzymestothesurfaceofamagneticbeadchooseDynabeads®Tosylactivated.Optimalantibodyorientationmakesthesebeadsanexcellentchoicefortheimmunoprecipitationofproteinsandproteincomplexes.

      IdealforImmunoprecipitationofproteincomplexes:
      LowbackgroundandcovalentlinkingofantibodiestothebeadsurfacemakeDynabeadsTosylactivatedanexcellentchoiceforImmunoprecipitationofproteinsandproteincomplexes(Co-Immunoprecipitation,Co-IP).Gentleandrapidmagneticconcentrationofthebeadsandshortincubationtimes(possIBLeduetofastsurface-basedbindingkinetics)makeDynabeadsTosylactivatedanexcellentchoicefortheimmunoprecipitationofhighly-lABIleorand/ortransient(short-lived)proteincomplexes.

      Ultrarapidprotocols:

      •Identifymembersofproteincomplexesinminutesratherthanhours
      •Temporalresolutionshortenoughtoidentifytransientandlabilecomplexes
      •Identifybindingpartnersthatcannotbeidentifiedwithlongerprotocols
      •Rapidprotocolsfurtherreducethealreadyultra-lowbackgroundbinding
      •Strongersignaltonoise-ratios

      Applications:
      •IPofproteinsandproteincomplexes
      •Couplefunctionalenzymestobeadsurfacefordownstreamassays
      •Couplepeptidestobeadsurfacetoidentifybinders

      Purifypeptides,proteinsandenzymesthatare:
      •Transientlystable
      •Structurallyintact
      •Temperaturelabile
      •Intheirnativeconformationandfunctional

      CouplingProcedureOutline:
      CovalentcouplingisperformedovernightbyincubatingthedesiredligandwiththeDynabeadsTosylactivated.LigandscommonlycoupledtoDynabeadsTosylactivatedincludepeptidesandproteins(e.g.antibodiesforImmunoprecipitationorCo-Immunoprecipitation).CouplingoccursatneutraltohighpHandat37°C.WerecommendcouplingatpH8.5-9.5,butforpHlabileligands,couplingcanbeperformedinanalternativebufferatpH7.4.

      Uponcompletionoftheligandcouplingstep,theactualDynabeadsTosylactivatedsurfacecoatingwillberenderedinert,resultinginlownon-specificbinding.

      AboutDynabeads:
      Dynabeadsarenon-porousmonodispersesuperparamagneticbeads.Theyarehighlymobileinsolutionenablingligandscoupledtothebeadstocontinuouslyinteractwiththeentiresamplevolume.Thesuperparamagneticbeadsarepulledtothetubewallsbytransferringthetubetoarackcontainingastrongmagneticfield(FromtherednavigationbarnavigateBrands>Dynal®>Magnets).Strongmagneticfieldsquicklypullthebeadstothetubewall,allowingforeasyandcompleteremovalofthesupernatantbyPipette.Washingstepsareperformedsimilarly.l

      BeadSurfaceCharacteristicsforDynabeadsTosylactivated:
      •p-toluene-sulfonyl(Tosyl)groups
      •Hydrophobic,pHNeutral
      •Covalentbindingbyprimaryamine(NH2)orsulphydryl(SH)groups

      Bindingcapacitypermilligrambeads:
      Variesdependingonligand,(e.g.5-10µgIgG)
      ForResearchUseOnly.Notforuseindiagnosticprocedures.

      Figures

      Specifications

      SurfaceFunctionality:Tosylactivated, p-Toluene-Sulfonyl
      ElutionVolume-min:5µl
      Form:BeadsinSUSPension
      HighThroughputCompatibility:HighThroughput-Compatible
      ProductLine:DYNAL®, Dynabeads®
      ProductSize:2mL
      SampleType(Specific):AnySampleType
      ShelfLife:36monthsfromdateofmanufacture
      ShippingCondition:RoomTemperature

      Contents&storage

      Storethesebeadsat2-8°C.
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