JB-4® is a water-soluble, 2-hydroxyethyl methacrylate (HEMA, also known as glycol methacrylate, GMA) based plastic resin kit used in the preparation of embedded samples for high resolution light microscopy (HRLM). JB-4 embedded tissues yield semi-thin sections (0.5µ-2µ) with excellent morphological structure preservation than paraffin processed tissues. JB-4 being a water-soluble media does not require dehydration to absolute alcohol except for dense, bloody, or fatty tissue specimens. Clearing agents such as xylene and chloroform are not required. JB-4 is excellent for small non-decalcified bone specimens, routine stains, special stains, and histochemical staining.
The polymerization of JB-4 is exothermic, which is easily controlled by polymerizing on ice or by using refrigeration at 4°C. Clear casts are obtained in 90 minutes or less. It has been successfully used in enzyme histochemistry, and auto-radiography.
JB-4® EmbeddingKit Contains: 800ml Solution A (Monomer) ; 30ml Solution B (Accelerator) ; 12g Catalyst (Benzoyl Peroxide, Plasticized).
For immuno procedures we recommend the Osteo-Bed Bone Embedding Kit (Catalog #17734). JB-4 is not recommended for immuno procedures as the glycol methacrylate cannot be removed from the section and may block antigen sites for most antibody reactions.
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